Linear Amplicon Sequencing

$5.00

Sale Sold out

Please input each sample name on a separate line. Please review our sample labeling and packing instructions if this is your first order with us.

Do you need us to do PCR with custom primers? I will provide purified amplicon DNA

How many reads do you need? Regular: ~3000 reads Big: ~6000 reads

🧬 Starting August 2025

Open to all customers!

Click here to see an example with SS3 amplicon validation

✅ No reference sequence is required to build the consensus.
✅ If you provide a reference, we’ll align your results and highlight any unwanted mutations.

🚚 Sample Pickup for Bay Area customers:

We offer free Dropbox pickup on Tuesdays and Thursdays.

Have your samples ready before 12:00 PM on a pickup day.

Samples received before 12:00 PM will be processed in the current week’s cycle!

📦 Customers mailing in samples from outside the Bay Area:

Ship your samples to us via any major carrier (UPS preferred).

You’ll receive a confirmation once your shipment is received.

Any samples received before Thursday 12:00 PM will be processed in the current week’s cycle!

⏱️ Turnaround Time (TAT)

Data delivery: Once a week, on Friday afternoons.

📄 Data Files Returned

About 0.4 Megabases (Mb) of NGS data (ONT Flow Cell, R10.4.1) will be generated for each amplicon.

Genbank file
FASTA File
GFF file
AB1 Chromatogram File
Breseq Analysis Report
Coverage Image
Per base data confidence report
Raw data (FASTQ file)

🧪 Sample Preparation Guidelines

Amplicon size: under 25 kbp.

DNA amount: 10 µL at > 50 ng/µL.

For accuracy, please measure concentration using Qubit (Nanodrop tends to be less accurate).

📂 Reference Files (optional)

If you want an alignment view:

After you place your order, you can go to seqresults.angstrominno.com to upload your references in GenBank (.gb or .gbk), Fasta (.fa or .fasta), or Snapgene format (.dna). Make sure you upload them to the corresponding Order ID for example: