Linear Amplicon Sequencing

🧬 Starting August 2025

Open to all customers!

Click here to see an example with SS3 amplicon validation

✅ No reference sequence is required to build the consensus.
✅ If you provide a reference, we’ll align your results and highlight any unwanted mutations.

🚚 Sample Pickup for Bay Area customers:

We offer free Dropbox pickup on Tuesdays and Thursdays.

Have your orders submitted by 3:00 PM on a pickup day. Print out your confirmation email with QR code and place in the dropbox. Our courier will come around 4:00 PM for pickup. Please let us know if you need later time.

 

📦 Customers mailing in samples from outside the Bay Area:

Ship your samples to us via any major carrier (UPS preferred).

You’ll receive a confirmation once your shipment is received.

Samples should arrive Tuesdays or Thursdays 4:00 PM Pacific for the overnight turnaround time!

 

⏱️ Turnaround Time (TAT)

Data delivery: Overnight from samples processed on Tuesdays or Thursdays.

 

📄 Data Files Returned

0.4-0.8 Megabases (Mb) of NGS data (ONT Flow Cell, R10.4.1) will be generated for each amplicon depending on the product variant you select at checkout. If you need significantly more, please contact us to discuss.

• Genbank file
• FASTA File
  
• .ab1 Chromatogram File
• Breseq Analysis Report
• Coverage Image
• Per base data confidence report
• Raw data (FASTQ file)

 

🧪 Sample Preparation Guidelines

Amplicon size: under 25 kbp. 

DNA amount: 10 µL at > 50 ng/µL.

For accuracy, please measure concentration using Qubit (Nanodrop tends to be less accurate).

 

📂 Reference Files (optional)

If you want an alignment view:

After you place your order, you can go to seqresults.angstrominno.com to upload your references in GenBank (.gb or .gbk), Fasta (.fa or .fasta), or Snapgene format (.dna). Make sure you upload them to the corresponding Order ID for example: 

If possible (not required), use the same file name for your references as the supplied sequencing sample name.