Amplicon Sequencing of SS3

The SS3 locus is a commonly used integration locus in E. coli engineering (Bassalo 2016). Here we validate the wildtype locus (containing the gRNA target sequence) using primers

 

Primer Name	Sequence
SS3_forward	GGC GGT GCA CAG CAA CAC G
SS3_reverse	CCG GTG ATA CAG CTT CAA TTC CAA CC

This amplifies a short product about 250 bp long.

Result when submitted to our Linear Amplicon service: https://seqresults.angstrominno.com/orders/AI-1272