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Whole Plasmid Sequencing

Whole Plasmid Sequencing

Regular price $5.00
Regular price Sale price $5.00
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Please input each sample name on a separate line. Please review our sample labeling and packing instructions if this is your first order with us.

What type of samples will you provide?

🧬 Starting August 2025

Open to all customers!Ā All plasmids under 25 kbp. No other restrictions

See your full plasmid sequence with automated annotations!
āœ… No reference sequence is required to build the consensus.
āœ… If you provide a reference, we’ll align your results and highlight any unwanted mutations.


🚚 Sample Pickup for Bay Area customers:

We offer free Dropbox pickup on Tuesdays and Thursdays.

Have your samples ready before 12:00 PM on a pickup day.

Samples received before 12:00 PM will beĀ processed in the current week’s cycle!

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šŸ“¦ Customers mailing in samples from outside the Bay Area:

Ship your samples to us via any major carrier (UPS preferred).

You’ll receive a confirmation once your shipment is received.

Any samples received beforeĀ Thursday 12:00 PM will be processed in the current week’s cycle!

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ā±ļø Turnaround Time (TAT)

Data delivery: Once a week, on Friday mornings.

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šŸ“„ Data Files Returned

About 0.4 Megabases (Mb) of NGS data (ONT Flow Cell, R10.4.1) will be generated for each plasmid.

  • Genbank file
  • FASTA File
  • GFF file
  • AB1 File
  • Breseq Analysis Report
  • Coverage Image
  • Interactive plasmid map
  • Per base data confidence report

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🧪 Sample Preparation Guidelines

Plasmid size: under 25 kbp.Ā 

DNA amount: 10 µL at 20-200 ng/µL range. Please inform us if your concentration is outside this range.Ā 

For accuracy, measure the concentration usingĀ Qubit (Nanodrop tends to be less accurate).

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šŸ“‚Ā Reference Files (optional)

If you want an alignment view:

After you place your order, you can go to seqresults.angstrominno.com to upload your references inĀ GenBank (.gb or .gbk), Fasta (.fa or .fasta), or Snapgene format (.dna). Make sure you upload them to the corresponding Order ID for example:Ā 

If possible (not required), use the same file name for your references as the supplied sequencing sample name.

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