Whole Plasmid Sequencing

$5.00

Sale Sold out

Please input each sample name on a separate line. Please review our sample labeling and packing instructions if this is your first order with us (especially if submitting plates).

What type of samples will you provide? I will provide purified plasmid DNA ($5/sample). Provide at least 10 μL of 20-200 ng/μl of DNA Angstrom will extract and sequence the plasmid DNA from agar colonies or pelleted liquid culture ($10/sample)

🧬 Starting August 2025

Open to all customers! All plasmids under 25 kbp. No other restrictions

See your full plasmid sequence with automated annotations!
✅ No reference sequence is required to build the consensus.
✅ If you provide a reference, we’ll align your results and highlight any unwanted mutations.

🚚 Sample Pickup for Bay Area customers:

We offer free Dropbox pickup on Tuesdays and Thursdays.

Have your orders submitted by 3:00 PM on a pickup day. Print out your confirmation email with QR code and place in the dropbox. Our courier will come around 4:00 PM for pickup. Please let us know if you need later time.

📦 Customers mailing in samples from outside the Bay Area:

Ship your samples to us via any major carrier (UPS preferred). Please send us the tracking number so we can plan for your samples. Samples need to be received by 4pm Pacific Time to be processed same day on Tuesday or Thursday. Samples received after 4pm will be processed in the next cycle (the next Tuesday or Thursday).

⏱️ Turnaround Time (TAT)

Your plasmid sequencing data will be ready overnight when submitted on Tuesdays and Thursday.

📄 Data Files Returned

You can see an example plasmid data report when you log into our Results Portal.

About 0.4 Megabases (Mb) of NGS data (ONT Flow Cell, R10.4.1) will be generated for each plasmid. The following data reports will be given for each sample:

Genbank file, FASTA File, .ab1 File, Breseq Analysis Report (reference based), Coverage Image, Interactive plasmid map, Per base data confidence report

Example shown here

🧪 How much plasmid sample should I send?

We need at least 10 µL at 20-200 ng/µL range. Please inform us if your concentration is outside this range.

For accuracy, measure the concentration using Qubit (Nanodrop tends to be less accurate).

📂 Reference Files (optional)

If you want an alignment view:

After you place your order, you can go to seqresults.angstrominno.com to upload your references in GenBank (.gb or .gbk), Fasta (.fa or .fasta), or Snapgene format (.dna). Make sure you upload them to the corresponding Order ID for example:

If possible (not required), use the same file name for your references as the supplied sequencing sample name.

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