Yeast Whole Genome Sequencing (less than 16 Mb)
Yeast Whole Genome Sequencing (less than 16 Mb)
Please input each sample name on a separate line. Please review our sample labeling and packing instructions if this is your first order with us (especially if submitting plates).
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𧬠Starting August 2025
Open to all customers!
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See your yeast whole genome sequence (WGS) with automated annotations!
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No reference sequence is required to build the consensus.
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If you provide a reference, weāll align your results and highlight any unwanted mutations.
Our pipeline is optimized for haploid sequences, but we also handle diploid and polyploid strains. Letās discuss your goals so we can tailor the process to your needs! Email info@angstrominno.com to start a discussion.
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š Sample Pickup for Bay Area customers:
We offerĀ free Dropbox pickupĀ onĀ Tuesdays and Thursdays.
Have your samples readyĀ before 12:00āÆPMĀ on a pickup day.
Samples received by 12:00āÆPM will be processed starting that day!
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š¦ Customers mailing in samples from outside the Bay Area:
Ship your samples to us via any major carrierĀ (UPS preferred).
Youāll receive a confirmation once your shipment is received.
Any samples received Tuesday or Thursday by 4:00āÆPM PacificĀ will be processed starting that day!
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ā±ļø Turnaround Time (TAT)
Data delivery:Ā About 2-3 days after samples have been received.
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š Data Files Returned
About 600 Megabases (Mb) of NGS data (ONT Flow Cell, R10.4.1) will be generated for each yeast sample.
- Interactive IGV plot (if reference is provided)
- Breseq Analysis Report (if reference is provided)
- Genbank file
- FASTA file
- GFF file (with annotations provided by MAKER)
- Raw data (FASTQ file)
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š§Ŗ Sample Preparation Guidelines
DNA amount:Ā 20āÆĀµLĀ atĀ >āÆ50āÆng/µL.
For accuracy, please measure concentration usingĀ QubitĀ (Nanodrop tends to be less accurate).
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šĀ Reference Files (optional)
If you want an alignment view:
After you place your order, you can go toĀ seqresults.angstrominno.comĀ to upload your references inĀ GenBank (.gb or .gbk), Fasta (.fa or .fasta), or Snapgene format (.dna). Make sure you upload them to the corresponding Order ID for example:Ā
If possible (not required), use theĀ same file name for your references as the supplied sequencing sample name.
