Plasmid Sequencing of pUC19, pACYC, and pET-28a(+)
In your results portal, you will find a demo order for Whole Plasmid Sequencing of three common plasmid vectors: pUC19, pACYC-Duet, and pET-28a(+).
When you open your order page, you will first see a summary of the plasmid sizes of all the de novo assemblies. You can hover over a sample bar to get more details.
We generate multiple reports per sample such as the interactive plasmid map, fasta file, genbank file, ab1 file, etc.
This is what the interactive map looks like for the first sample (pUC19):
Here is the read length histogram with a sharp peak at the respective size for pUC19 (~2.6 kb).
Many of our customers like this interactive IGV view that aligns the raw nanopore reads with the auto-annotated de novo assembly.
We can also do reference based alignment. Here the public sequence for pUC19 was uploaded. After alignment, you can see there are no mutations and it is a 100% match.
Each sample that has a successful assembly, will also have a "Quality Badge" score. This provide a quick view if the sample met important criteria regarding coverage, purity, and per base confidence.
