WGS of BL21(DE3)

 

The example below shows the whole genome sequencing result for BL21(DE3), a host frequently used for T7 promoter expression. 

For the analysis below, the updated 2024 reference sequence NZ_CP053602.1 was used. 

Interactive IGV weblink:

https://igv.angstrominno.com/?sessionURL=/data/AI-1045_1_BL21_DE3/session.json#

Screenshot below shows a random mutation at cnoX gene

Assembly (FASTA format)

BL21_DE3.fasta

GenBank File

BL21_DE3.gbk

Screenshot below of the annotated map

BRESEQ (showing all the mutations relative to the reference)

Raw FASTQ file (typically around 200 Mb)

BL21_DE3.fastq.gz