{"product_id":"cultureprep","title":"CulturePrep: Whole Plasmid Sequencing from liquid cultures","description":"\u003cp\u003e\u003cstrong\u003eNote:\u003cspan\u003e \u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003efor CulturePrep we recommend to grow the culture in LB media. Although TB can work, the increase buffer capacity can affect the results which requires us to use a modified protocol. \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0694\/8598\/5991\/files\/cultureprep.png?v=1783394017\" alt=\"\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2 data-start=\"237\" data-end=\"347\"\u003e\n\u003cstrong data-start=\"237\" data-end=\"261\"\u003e🧬 \u003c\/strong\u003eHigh Quality Data \u0026amp; Amazing Price \u003c\/h2\u003e\n\u003cp\u003eAll plasmids from 0 to 30 kilobase pairs (kbp). No other restrictions\u003c\/p\u003e\n\u003cp\u003e\u003cstrong data-start=\"349\" data-end=\"411\"\u003eSee your full plasmid sequence with automated annotations!\u003c\/strong\u003e\u003cbr data-start=\"411\" data-end=\"414\"\u003e✅ No reference sequence is required to build the consensus.\u003cbr data-start=\"473\" data-end=\"476\"\u003e✅ If you provide a reference, we’ll align your results and highlight any unwanted mutations.\u003c\/p\u003e\n\u003ch3 data-start=\"865\" data-end=\"893\"\u003e\u003cbr\u003e\u003c\/h3\u003e\n\u003ch3 data-start=\"865\" data-end=\"893\"\u003e🚚 Sample Pickup for Bay Area customers:\u003c\/h3\u003e\n\u003cp\u003eWe offer \u003cstrong data-start=\"931\" data-end=\"954\"\u003efree Dropbox pickup\u003c\/strong\u003e on \u003cb\u003eMonday to Friday\u003c\/b\u003e\u003c\/p\u003e\n\u003cp\u003eHave your orders submitted by \u003cstrong\u003e3\u003c\/strong\u003e\u003cstrong data-start=\"1014\" data-end=\"1033\"\u003e:00 PM\u003c\/strong\u003e on a pickup day. Print out your confirmation email with QR code and place in the dropbox. Our couriers will typically come after 4pm, varies by location. Email us ( \u003ca href=\"mailto:info@angstrominno.com\" rel=\"noopener\" target=\"_blank\"\u003einfo@angstrominno.com\u003c\/a\u003e ) to know your pickup time if you are a new customer.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cstrong data-start=\"1141\" data-end=\"1176\"\u003e📦 Customers mailing in samples from outside the Bay Area:\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003eShip your samples to us via any major carrier \u003cstrong\u003e(UPS preferred).\u003c\/strong\u003e Please send us the tracking number so we can plan for your samples. Samples need to be received by 4pm Pacific Time to be processed same day. Samples received after 4pm will be processed in the next processing day.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3 data-start=\"575\" data-end=\"600\"\u003e⏱️ Turnaround Time (TAT)\u003c\/h3\u003e\n\u003cp\u003eYour plasmid sequencing data will be ready overnight Monday to Friday.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003e📄 Data Files Returned\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003eYou can see an example plasmid data report when you log into our \u003ca href=\"http:\/\/seqresults.angstrominno.com\"\u003eResults Portal\u003c\/a\u003e.\u003c\/p\u003e\n\u003cp\u003eAbout 0.4 Megabases (Mb) of NGS data (ONT Flow Cell, R10.4.1) will be generated for each plasmid. The following data reports will be given for each sample:\u003c\/p\u003e\n\u003cp\u003eGenbank file, FASTA File, .ab1 File, Breseq Analysis Report (reference based), Coverage Image, Interactive plasmid map, Per base data confidence report\u003c\/p\u003e\n\u003cp\u003eExample shown here\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/seqresults.angstrominno.com\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0694\/8598\/5991\/files\/plasmid_data_6658b10a-1f2e-4f17-8611-7ecd310e4dd4.png?v=1758301470\" alt=\"\"\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003e🧪 How much plasmid sample should I send?\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003eWe need at least \u003cstrong data-start=\"1476\" data-end=\"1485\"\u003e10 µL\u003c\/strong\u003e at \u003cstrong data-start=\"1489\" data-end=\"1503\"\u003e20-200 ng\/µL range\u003c\/strong\u003e. Please inform us if your concentration is outside this range. \u003c\/p\u003e\n\u003cp\u003eFor accuracy, measure the concentration using \u003cstrong data-start=\"1560\" data-end=\"1569\"\u003eQubit\u003c\/strong\u003e (Nanodrop tends to be less accurate).\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3 data-start=\"1614\" data-end=\"1644\"\u003e📂 Reference Files (optional)\u003c\/h3\u003e\n\u003cp\u003eIf you want an alignment view:\u003c\/p\u003e\n\u003cp\u003eAfter you place your order, you can go to \u003ca href=\"https:\/\/seqresults.angstrominno.com\/\" rel=\"noopener\" target=\"_blank\"\u003eseqresults.angstrominno.com\u003c\/a\u003e to upload your references in \u003cstrong data-start=\"1707\" data-end=\"1725\"\u003eGenBank (.gb or .gbk), Fasta (.fa or .fasta), or Snapgene format (.dna)\u003c\/strong\u003e. Make sure you upload them to the corresponding Order ID for example: \u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0694\/8598\/5991\/files\/reference_upload.png?v=1753149125\" alt=\"\" width=\"328\" height=\"244\"\u003e\u003c\/p\u003e\n\u003cp\u003eIf possible (not required), use the same file name for your references as the supplied sequencing sample name.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Angstrom Innovation","offers":[{"title":"Dense liquid culture 200+ μL (overnight process) \/ LB (recommended)","offer_id":46735317303495,"sku":null,"price":15.0,"currency_code":"USD","in_stock":true},{"title":"Dense liquid culture 200+ μL (overnight process) \/ TB","offer_id":46735317336263,"sku":null,"price":15.0,"currency_code":"USD","in_stock":true},{"title":"Dense liquid culture 200+ μL (overnight process) \/ Other (please communicate with us via email)","offer_id":46735317369031,"sku":null,"price":15.0,"currency_code":"USD","in_stock":true},{"title":"Colony inoculated in 200+ μL media (2 day process) \/ LB (recommended)","offer_id":46735501590727,"sku":null,"price":15.0,"currency_code":"USD","in_stock":true},{"title":"Colony inoculated in 200+ μL media (2 day process) \/ TB","offer_id":46735501623495,"sku":null,"price":15.0,"currency_code":"USD","in_stock":true},{"title":"Colony inoculated in 200+ μL media (2 day process) \/ Other (please communicate with us via email)","offer_id":46735501656263,"sku":null,"price":15.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0694\/8598\/5991\/files\/ChatGPTImageJul6_2026_08_26_08PM.png?v=1783394798","url":"https:\/\/sequencing.angstrominno.com\/products\/cultureprep","provider":"Angstrom Innovation","version":"1.0","type":"link"}